Dietary low-phytate mutant-M 955 barley grain alters phytate degradation and mineral digestion in sheep fed high-grain diets

Greater production demands for ruminants require increased dietary inclusion of high-energy feeds. Grains and oil seeds are most commonly used to enhance diet energy density. However, use of such feeds proportionally increases the amount of dietary phytate phosphorus (P), which the ruminant may not be able to fully utilise. Our objectives for this study were to determine the extent of phytate degradation and mineral digestion in wethers fed high-grain diets consisting of either a non-mutant or low-phytate mutant barley grain. In two separate experiments, mature Columbia wethers (n= 7) fitted with rumen and duodenal cannulas and Columbia×Polypay wether lambs (n= 8) were individually fed one of two finishing diets formulated with either non-mutant Harrington (HARR) variety or lowphytate mutant-M 955 (M955) barley grains. Total-P intake was similar (P=0.46–0.70) between the M955 and HARR treatments for mature (5756 and 5550 mg/day, respectively) and lamb (5207 and 4894 mg/day, respectively) wethers. Dietary water-soluble P was 3.6 times greater in M955 versus Abbreviations: ARS, Agricultural Research Service; Ca, calcium; Fe, iron; HARR, Harrington variety barley grain; M955, mutant-M 955 variety barley grain; Mg, magnesium; P, phosphorus; USDA, United States Department of Agriculture; Zn, zinc ∗ Corresponding author. Tel.: +1 208 374 5306; fax: +1 208 374 5582. E-mail address: [email protected] (J.B. Taylor). 0377-8401/$ – see front matter © 2006 Elsevier B.V. All rights reserved. doi:10.1016/j.anifeedsci.2006.11.005 14 A.B. Leytem et al. / Animal Feed Science and Technology 138 (2007) 13–28 HARR diets and phytate P was 11 times greater in HARR versus M955 treatment diets. Apparent total-P digestion was similar between M955 and HARR treatments (P=0.52–0.69). More monoester P was identified in the duodenal chyme of mature wethers fed HARR treatment diet, presumably due to incomplete hydrolysis of phytate P in the rumen. Feeding M955, compared to HARR, treatment diet resulted in greater (P<0.05) apparent partial-tract digestion of calcium (Ca) and total-tract digestion of iron (Fe), magnesium (Mg), and zinc in mature wethers and apparent total-tract digestion of Mg and Fe and retention of Ca, Fe, and Mg in wether lambs. These results indicate that phytate in diets formulated with Harrington variety barley grain may not be fully digested in the rumen. Subsequent passage of partially digested phytate from the rumen may interfere with mineral digestion in wethers fed high-grain diets. © 2006 Elsevier B.V. All rights reserved.